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Clomiphene Citrate Challenge Test (CCCT) or Clomid Challenge Test (CCT)

The Clomid Challenge test is a test of ovarian reserve, or ovarian function study.  The clomiphene challenge test is used to assess the ability of the ovaries to respond to hormone signals. This helps assess ovarian reserve and aids with counseling patients on options for fertility treatment.  There is some variability in how different programs request a CCCT.  Below is a general guideline of the most common approach.

The Clomid Challenge Test has been replaced by more advanced forms of ovarian reserve testing.


Day 1: Onset menses

Day 3: Blood draw

  • Serum Estradiol (E2)
  • Serum FSH

Day 5-9: Clomid 100mg DAILY (two 50 mg tablets)

Day 10: Blood draw

  • Serum FSH
  • +/- Serum Estradiol (E2)
  • +/- Serum LH


Expected Values

CD (cycle day) 3

E2 value 65-70

FSH value below 10

CD (cycle day) 10 or 11

LH value below 10

FSH value below 10


Women with elevated FSH levels may demonstrate diminished ovarian reserve.